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Lightly, poly(A new)+ RNA was singled out simply by oligo-dT variety, fragmented, as well as helped by phosphatase and also polynucleotide kinase to mend your ends. RNA plugs (3�� as well as 5��) ended up after that sequentially ligated to the RNA fragments along with change transcribed using a for beginners complementary towards the 3��?linker. The libraries ended up next PCR amplified as well as

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