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Lightly, poly(A)+ RNA was remote by simply oligo-dT choice, fragmented, and treated with phosphatase as well as polynucleotide kinase to fix your finishes. RNA adapters (3�� as well as 5��) ended up next sequentially ligated towards the RNA pieces along with change transcribed utilizing a federal government contrasting on the 3��?linker. The libraries were then PCR zoomed along with sequenced in b

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